Isolation of actinobacillus seminis from rams in the united kingdom. Clinical infections are usually unresponsive to treatment, and because infected animals are of considerable financial value, economic losses. Microbial identification databases for biolog systems. Emended description of porcine pasteurella aerogenes. Actinobacillus seminis isolated from cattle sciquest. Pcr methods for rapid identification and characterization of. Actinobacillosis refers to a group of diseases caused by gramnegative coccobacilli in the genus actinobacillus. The aim of this study was to improve the definition and identification of a group of veterinarily important bacteria referred to as the pasteurella aerogenespasteurella mairiiactinobacillus rossii complex. This bacterium is also known to affect other organisms, since it has been isolated from.
The species is not recognised today and is almost certainly what we now term haemophilus somnus. Pdf isolation of actinobacillus seminis from a goat with. Although there are 22 different bacterial species in this genus, only four a pleuropneumoniae, a suis, a equuli, and a lignieresii are frequently associated with disease in animals. The isolates of a seminis showed similar phenotypic profiles and electrophoretic protein patterns to the type strain of a seminis but were distinct from histophilus ovis previously isolated from rams with epididymitis in scotland. Isolation and characterization of actinobacillus seminis strains from. Biological characterization of actinobacillus species and. The bacteria responsible for a particular bacterially induced disease is contacted with a detergent so as to extract from the bacterial cells the antigens responsible for eliciting a protective immune response against the disease. Genome sequence of actinobacillus seminis strain atcc. Pdf the present study reports the first isolation of actinobacillus seminis from a goat in brazil. Actinobacillus seminis as a cause of abortion in a uk sheep flock. Changes frequently observed include abscess in the left epididymis tail, which was the site of inoculation figures 5a and 5b, and an increase in size of inguinal and iliac lymph nodes table 2. Rep and ericpcr could be used for strain differentiation in epidemiological studies of a.
Actinobacillus seminis, a commensal of ovine and caprine reproductive organs, is able to induce epididymitis in the small ruminants that it infects. Actinobacillus seminis groelhomologous protein agglutinates. The present invention relates to novel bacterial extract vaccines for use in veterinary medicine and to novel methods for preparing such vaccines. In this work, we characterised two protein bands of approximately 150 kda and 65 kda. Pdf actinobacillus seminis as a cause of abortion in a. Deoxyribonucleic acid relationships of histophilus ovis. Haemophilus somnus possesses two systems for acquisition of. It was first isolated in the united kingdom in 1991 and in one survey was found to be present in the semen of 19% of infertile rams. Five types were distinguished by reppcr, and nine types were distinguished by ericpcr. Speciesspecific multiplex pcr for the diagnosis of. Clinical infections are usually unresponsive to treatment, and because infected animals are of considerable financial value. Dec 21, 2019 actinobacillus seminis is a gramnegative bacterium of acinobacillus pasteurellaceae family that is involved in ovine epididymitis. The most common causative agents of these infections are brucella ovis, actinobacillus seminis, and histophilus somni.
Actinobacillus seminis infection in sheep in the republic of. Determination of pathological changes in the reproductive track, igg, igm and iga antibodies in blood, seminal plasma and smegma of rams inoculated with actinobacillus. Left testis is decreased in volume and tunica vaginalis is thickened and firmly adhered to the testis. An outbreak of epididymitis and orchitis associated with actinobacillus seminis infection is reported. Morphological, biochemical, antigenic, and cytochemical. Infected rams were found to have abscessed testes and epididymi and. Nine groups of four 18 to 24monthold rams were inoculated with actinobacillus seminis by the following routes. Speciesspecific multiplex pcr for the diagnosis of brucella. Dna fingerprinting of haemophilus somnus, histophilus ovis. Isolation of actinobacillus seminis from a goat with clinical epididymoorchitis in brazil. Analysis of the protein profiles and outer membrane antigens of a. A fouryearold moxoto breeding goat in a flock of 70 goats and 65 sheep reared together in the county of patos, semiarid region of northeastern brazil, showed clinical signs of unilateral orchitis and epididymitis. In the outbreak, both young unmated rams and mature breeding rams were affected.
Twentyfour isolates of actinobacillus seminis were typed by pcr ribotyping, repetitive extragenic palindromic element repbased pcr, and enterobacterial repetitive intergenic consensus ericbased pcr. Actinobacillus seminis definition of actinobacillus. In immunodiffusion tests, hh group bacteria had at least two antigens common to. Pcr ribotyping produced the simplest pattern and could be useful for identification of a. Actinobacillus seminis in sheep actinobacillus seminis in sheep baynes, i. The bacteria are facultatively anaerobic or aerobic, capable of fermenting carbohydrates without production of gas, and of reducing nitrates. The aim of this study was to develop a multiplex pcr assay for simultaneous detection of brucella ovis, actinobacillus seminis, and. Speciesspecific multiplex pcr for the diagnosis of brucella ovis. Actinobacillus seminis is a gramnegative bacterium, a member of the pasteurellaceae family, and a causal agent of epididymitis and testicular lesions in sheep, producing low rates of fertility and abortions 1, 2 and consequent economic losses to the sheep industry. Yaeger, dvm, phd tinobacillus suis infection in swine has generally been associated with septicemia and death in suckling and reently weaned pigs.
The objective of this study was to demonstrate that a field isolate of actinobacillus seminis as8c will adhere to epithelial cells and that this adhesion can be inhibited by pretreating the bacteria with mouse serum containing polyclonal antibodies poabs prepared against this isolate. In immunodiffusion tests, hh group bacteria had at least two. These proteins crossreacted with a polyclonal serum against gallibacterium anatis hemagglutinin and with a polyclonal serum from sheep with. A model for demonstrating the adhesion of actinobacillus. Actinomyces actinomycetemcomitans dr harshavardhan patwal. Us4981685a bacterial extract vaccines for veterinary. Four of the rams had abnormal semen and three were also infertile. One of the most common forms seen by veterinarians is mouth actinobacillosis of cattle, due to actinobacillus lignieresii. Experimental transmission of actinobacillus seminis. Analysis of pcramplified genomic fragments showed t. Shape cells spherical, oval or rodshaped staining gramnegative. This article has been scanned in colour with a hp scanjet 5590.
Actinobacillus seminis was isolated from the semen of five rams on four farms. Identification of an immunogenic protein of actinobacillus seminis that is present in microvesicles. Since then, the prevalence of ovine epididymitis due to a. The aim of this study was to develop a multiplex pcr assay for simultaneous detection of brucella ovis, actinobacillus seminis, and histophilus. Overview of actinobacillosis generalized conditions. Actinobacillus seminis and related strains of the socalled gramnegative pleomorphic organisms also induce epididymitis. Actinobacillus seminis was isolated in pure and mixed culture from the urethra in two yearlings, one which was infected and one which was not, while bacilli were isolated from one uninfected ram. Actinobacillus mallei definition of actinobacillus. Actinobacillus seminis is a gramnegative bacterium of acinobacillus pasteurellaceae family that is involved in ovine epididymitis. Identification of an immunogenic protein of actinobacillus. Actinobacillus seminis as a cause of ram infertility in. View or download all content the institution has subscribed to. This usually occurs at puberty, when there are elevated levels of gonadotrophin releasing hormones. Actinobacillus seminis infection of the head of epididymis of a 4yearold suffolk ram.
A 16s23s ribosomal spacer from a haemophilus parainfluenzae rrn locus was cloned and sequenced. Abstract the organism commonly referred to as actinobacillus seminis is an accepted cause of suppurative epididymitis of rams worldwide, it has also been associated with polyarthritis, myocarditis and posthitis in sheep, and isolated from aborting ewes, goat does and their foetuses. Actinobacillus arthritidis actinobacillus equuli subsp. Actinobacillus seminis is a gramnegative bacterium of the pasteurellaceae family that is involved in ovine epididymitis. The most prominent symptom is the swelling of the tongue that protrudes from the mouth and is very hard at palpation wooden tongue. The first page of the pdf of this article appears above.
Pdf sheep epididymitis by actinobacillus seminis in the state of. Genome sequence of actinobacillus seminis strain atcc 15768. Clinical and pathological changes in rams experimentally. Actinobacillus seminis as a cause of ram infertility in kenya. Actinobacillus is a genus of gramnegative, nonmotile and nonsporeforming, oval to rodshaped bacteria occurring as parasites or pathogens in mammals, birds, and reptiles. Actinobacillus seminis infection in sheep in the republic.
The organisms are capable of infecting cattle and other domestic animals and, rarely, are associated with human infection. Cloning and sequencing of a 16s23s ribosomal spacer from. The serological relationships between 4 strains of histophilus ovis, the neotype strain of actinobacillus seminis and brucella ovis were examined using a cross. Biochemical properties of aa slots 5 biochemical characters in 6 reference strains and strains of aa freshly isolated from the oral cavity. Diagnosis is confirmed by sequential ultrasonographic examination of the pampiniform plexus, testicle and tail of the epididymis undertaken as the 5 mhz linear scanner head is moved distally. These proteins crossreacted with a polyclonal serum against gallibacterium anatis hemagglutinin and with a polyclonal serum from sheep with epididymitis. Isolation of actinobacillus seminis from rams in the. We describe the isolation of actinobacillus lignieresii and an a. Actinobacillus seminis an overview sciencedirect topics. Epididymitis caused by actinobacillus seminis histophilus ovis gramnegative pleomorphic organisms is a major cause of ram infertility in the uk. All structured data from the file and property namespaces is available under the creative commons cc0 license.
Files are available under licenses specified on their description page. Systemic clinical signs were minimal and were confined primarily to the inoculation sites and the. Clinical examination revealed mild tachypnoea and hyperpnoea and marked tachycardia and pallor. N o evidence of clinical disease was apparent in the ewe flock and we were unable to isolate the organism from nine. Identification of two adhesins of actinobacillus seminis.
These organisms have mainly been isolated from the reproductive and intestinal tracts of pigs and in most cases have been considered as opportunistic pathogens. Expression of tf receptors, as evaluated by solidphase binding assays, required the organisms to be grown under ironrestricted conditions in the presence of tf. Isolation of actinobacillus seminis from a goat with clinical. Actinobacillus seminis is a gramnegative bacterium of the pasteurellaceae family that is a natural inhabitant of the preputial mucosa of sheep and goats. Pdf actinobacillus seminis as a cause of abortion in a uk. Mar 21, 20 infectious ovine epididymitis results in substantial economic losses worldwide due to reproductive failure and culling of breeders. Haemophilus somnus strain 649 was found to acquire iron from ovine, bovine, and goat transferrins tfs.
Actinobacillus seminis 2 pv actinobacillus succinogenes 2 pv actinobacillus suis 2 pv actinobacillus ureae 2 old name pasteurella ureae actinobaculum. It is associated with epididymitis of sheep references. Actinobacillus seminis is a pleomorphic, gramnegative bacillus that is a wellknown cause of epididymitis in australian rams baynes and simmons, 1960, and has been documented in the united states of america, south africa, new zealand, hungary, patagonia, the united kingdom, turkey, kidney, and spain. Aug 12, 2019 linear integrated circuits by ramakant gaikwad pdf opamps and linear integrated circuits on amazon. So far, only low levels of genetic relatedness with actinobacillus seminis and currently recognized members of the family pasteurellaceae have been detected. Pathology of experimental histophilus ovis infection in sheep. Actinobacillus seminis actinobacillus succinogenes actinobacillus suis actinobacillus ureae actinomyces bovis.
Endocarditis and ventricular wall abscessation in a. Actinobacillus porcitonsillarum is a gramnegative rod belonging to the pasteurellaceae family, which is regularly isolated from the tonsils of healthy pigs and phenotypically resembles actinobacillus pleuropneumoniae, the causative agent of porcine pleuropneumonia, which is associated with high economic burdens in the pig industry. Pdf deoxyribonucleic acid relationships of histophilus. Pdf isolation of actinobacillus seminis from a goat with clinical. Evidence from numerical taxonomic analysis and dnadna hybridization supports the proposal of new species in the genera actinobacillus and pasteurella. Morphology, biochemical reactions, pigmentation, antigens, and cell envelope proteins were examined in 12 strains of haemophilus somnus, haemophilus agni, histophilus ovis, and actinobacillus seminis. Competition binding assays revealed the presence of two distinct tfbinding receptor systems, one specific for bovine tf and the. A fouryearold vasectomised herdwick ram presented with a twoday history of inappetence, lethargy, weakness and exercise intolerance. Download fulltext pdf deoxyribonucleic acid relationships of histophilus ovishaemophilus somnus, haemophilus haemoglobinophilus, and actinobacillus seminis.
Ram breeding soundness some common scrotal and testicular. Comparative genomics of the first and complete genome of. Actinobacillus salpingitidis actinobacillus seminis actinobacillus ureae actinobacillus sp. Actinobacillus seminis infection in rams constitutes a spectrum of. Summary eighteen strains of actinobacillus seminis isolated from ovine semen samples and epididymitis were characterized according to their cultural and. Infectious ovine epididymitis results in substantial economic losses worldwide due to reproductive failure and culling of breeders. The bacteria are facultatively aerobic or anaerobic, capable of fermenting carbohydrates without production of gas, and of. Dear sir, in a paper submitted for publication in the australian veterinary journal, we have reported serological evidence of actinohocilluj seminis infection in 23 ewes from a border leicester flock. Semen obtained from infected rams yielded actinobacillus seminis in pure or mixed culture in five of the six clinically infected rams.
They are very similar in cultural and biochemical characteristics and these phenotypic characteristics are highly variable between strains of each species. Looking for a protein specific to this species, we determined the protein profile of subcellular fractions of a. Pcr methods for rapid identification and characterization. Thoracic ultrasonography revealed thickening of the right ventricular wall and increased pericardial and peritoneal fluid. Isolation of actinobacillus seminis from rams in the united. On the other hand, one of the haemophilus agni strains studied hoerlein strain m65043 was included in the species actinobacillus seminis dna binding value, 91%. Eight similar rams were left uninoculated as controls. Research article open access speciesspecific multiplex. It was not until 1960 that baynes and simmons 1960 reported another organism which could be included in the genus. Actinobacillus seminis in sheep, australian veterinary. Ovine epididymitis also occurs as a consequence of infection with actinobacillus seminis and histophilus ovis walker et al. Actinobacillusspecies and their role in animal disease. This was actinobacillus seminis isolated from epididymitis in three rams in australia. Actinobacillus seminis is a common cause of ovine epididymitis and ram infertility throughout the world 2, 6, 10, 21.
Jun 22, 2019 actinobacillus seminis, a commensal of ovine and caprine reproductive organs, is able to induce epididymitis in the small ruminants that it infects. The present study reports the first isolation of actinobacillus seminis from a goat in brazil. Infection with these organisms is common in virgin rams and ramlambproducing flocks bagley et al. Actinobacillosis is a zoonotic disease caused by actinobacillus it is more commonly associated with animals than with humans. Actinobacillus minor actinobacillus porcinus actinobacillus indolicus. Aug 12, 2016 taxonomy of aa belongs to pasteurellaceae pasteurella haemophilus actinobacillus genus. A clinical palpation and semen smear examination of 647 rams submitted to the regional veterinary laboratory during 1967 revealed that 42 6,5% of these animals had clinical epididymitis or orchitis, 6 0,9% showed other types of genital lesions and 98.
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